SolisFAST® Master Mix with UNG

A 5x-concentrated master mix from Solis BioDyne formulated for fast singleplex and multiplex endpoint PCR assays. The mix combines an in-silico designed SolisFAST® DNA Polymerase with the Stability TAG technology and an optimized buffer, providing increased tolerance to inhibitors. It supports amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda or plasmid DNA) and up to 3 kb from complex templates such as human genomic DNA. Reaction set-up requires only the addition of primers, nuclease-free water and DNA template.

Using a fast PCR cycler (Eppendorf® Mastercycler® X50s, 10 °C/sec ramp rate) with the recommended fast cycling program — initial activation 1 min at 98 °C, denaturation 5 sec at 98 °C, annealing/extension 20 sec at 60 °C, 25 cycles — a 515 bp HIRA fragment from human gDNA was amplified in 20 minutes. The mix is shipped ice-free. Variants containing UNG help avoid carry-over contamination and have been shown not to affect Sanger sequencing results following a 24-hour room-temperature incubation of PCR products.

The product is a colorless liquid with a pH of 7.0–9.0. It is classified as not hazardous under Regulation (EC) No 1272/2008, is stable under normal conditions, and is not regulated for land (ADR/RID) or air (ICAO/IATA) transport, being considered non-hazardous for transport. Solis BioDyne recommends long-term storage at –20 °C in the original container; the SolisFAST® Master Mixes with UNG remain stable for 1 month at 25 °C. The product is intended for Research Use Only.