SolisFAST® Master Mix

From Solis BioDyne, this 5x-concentrated ready-to-use solution is designed for fast singleplex and multiplex endpoint PCR assays. The premix contains all reagents required for hot-start PCR, so users only need to add primers, nuclease-free water, and DNA template.

The mix combines an in-silico designed SolisFAST DNA Polymerase with the Stability TAG system, an optimized buffer, and increased tolerance to inhibitors. It amplifies fragments up to 5 kb from low-complexity templates such as lambda or plasmid DNA, and up to 3 kb from complex templates such as human genomic DNA. Performance is demonstrated in singleplex and multiplex assays, with results achievable in around 20 minutes on fast cyclers.

The product is stable at room temperature for up to two months, supports ice-free shipping and reaction set-up, and is suitable for Sanger sequencing applications. Carry-over contamination can be avoided by selecting the corresponding UNG-containing variant.