SolisFAST® Master Mix, Ready to Load

A 5x-concentrated hot-start master mix from Solis BioDyne, designed for fast singleplex and multiplex endpoint PCR assays. The Ready To Load format supports a streamlined workflow. Combining a novel in-silico designed SolisFAST® DNA Polymerase with the Stability TAG technology, the mix delivers increased tolerance to inhibitors and reliable amplification. It supports fragments of up to 5 kb from low-complexity DNA templates such as lambda or plasmid DNA, and up to 3 kb from complex templates like human genomic DNA.

Fast cycling conditions include an initial activation of 1 minute at 98 °C, denaturation for 5 seconds at 98 °C, and annealing/extension for 20 seconds at 60 °C over 25 cycles, delivering results in 20 minutes on compatible fast cyclers. The mix is 2 months stable at 25 °C, enabling ice-free shipping and room-temperature reaction set-up — only primers, nuclease-free water, and DNA template need to be added. According to the manufacturer's MSDS, the liquid product is green in appearance with a pH of 7.0–9.0, is not classified as hazardous under EC Regulation 1272/2008, and is intended for Research Use Only. Recommended storage is at -20 °C in the original container.