RiboGrip® RNase Inhibitor (220 U/µl)

An in silico-designed protein-based ribonuclease inhibitor from Solis BioDyne, supplied at a high concentration formulation of 220 U/µl. The active protein is a unique chimeric molecule of mammalian origin, expressed in E. coli and purified using state-of-the-art protein purification methods. It inhibits ribonuclease activity by forming a strong noncovalent bond with target RNases in a non-competitive mode at a 1:1 ratio.

Enhanced stability is conferred by Solis BioDyne's proprietary and patented Stability TAG technology, which enables room temperature shipping and tolerance to demanding assay conditions — the inhibitor remains functional after incubation at 60 °C for up to 60 minutes, or at room temperature (25 °C) for up to 1 month. The product provides strong inhibition of eukaryotic RNases, including RNase A, B, and C, and offers efficient protection of RNA at low DTT concentrations. In 1-step RT-PCR experiments, 1.37 U/μl of the inhibitor demonstrated efficient inhibition of RNase A across the entire range of 100–2000 pg.

Typical applications include first-strand cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP, in vitro transcription and translation, RNA isolation and purification, and RNA sequencing. The inhibitor is compatible with reverse transcriptases (including FIREScript® and SOLIScript®), Taq Polymerase, and Bsm Polymerase, making it a flexible fit for one-step RT-(q)PCR assays and complex RNA templates requiring higher denaturation or reaction temperatures up to 60 °C. A glycerol-free formulation is also available for applications involving lyophilisation or air-drying.