RiboGrip® Glycerol-Free RNase Inhibitor (220 U/µl)

Supplied at a concentration of 220 U/µl, this glycerol-free RNase inhibitor from Solis BioDyne is an in silico-designed, protein-based ribonuclease inhibitor that protects RNA integrity in demanding molecular biology workflows. The chimeric protein of mammalian origin, expressed in E. coli, inhibits ribonuclease activity by forming a strong noncovalent bond in a non-competitive mode at a 1:1 ratio, providing strong protection against eukaryotic RNases including RNase A, B, and C.

Enhanced with the company's proprietary and patented Stability TAG technology, the RiboGrip® Glycerol-Free RNase Inhibitor tolerates up to 60 minutes at 60 °C or up to 1 month at room temperature (25 °C), supporting dry-ice-free shipping and use in assays requiring high incubation temperatures. The glycerol-free composition is well suited for lyophilization and air-drying applications. Recommended uses include first-strand cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP, in vitro transcription and translation, RNA isolation and purification, and RNA sequencing. The inhibitor is compatible with reverse transcriptases such as FIREScript® and SOLIScript®, as well as Taq and Bsm Polymerase, and remains effective at low DTT concentrations.

Performance data show that 1.37 U/μl of the inhibitor provided effective inhibition of RNase A across a 100–2000 pg range within a 1-step RT-PCR reaction, without compromising target amplification. Third-party handling notes list room temperature transportation and -20 °C storage.