FIREScript® RT cDNA synthesis MIX with Random primers (100 rxn)

A cDNA synthesis mix by Solis BioDyne delivers first-strand cDNA synthesis from a three-tube format that combines FIREScript® Enzyme Mix, 10x RT Reaction Premix with Random primers (containing DTT and dNTPs), and nuclease-free water. The FIREScript® Reverse Transcriptase is a genetically modified MMLV-based enzyme with full RNase H activity, stabilized by a patented modification in the polypeptide structure referred to as the Stability TAG.

The enzyme operates across 37°C to 60°C, with an optimum at 50°C, and is compatible with oligo (dT), random, and gene-specific primers. Reaction time ranges from 5 to 30 minutes, and the enzyme can transcribe up to 8.9 kb RNA sequences, generating full-length cDNA of at least 8 kb from total RNA amounts as low as 0.01 ng. One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(rA)·oligo(dT) in a 50 µl reaction. The mix is stable at room temperature (15–25°C) for 1 month, at 4°C for 6 months, and supports up to 30 freeze-thaw cycles, with routine storage recommended at –20°C.

Applications include gene expression analysis, disease diagnosis, genomic analysis, and structural studies of mRNA. The product is manufactured under ISO 9001 and ISO 13485 certified quality management and is supplied for research use only.