RiboGrip® Glycerol-Free RNase Inhibitor (220 U/µl)

A protein-based ribonuclease inhibitor from Solis BioDyne, supplied as a glycerol-free liquid formulation suited to lyophilization and air-drying workflows. The inhibitor is a chimeric protein of mammalian origin, expressed in E. coli and purified by state-of-the-art protein purification methods, with enhanced stability supported by the manufacturer's patented Stability TAG technology. It forms a 1:1 noncovalent bond in a non-competitive mode with eukaryotic RNases (including RNase A, B and C), protecting RNA from degradation.

Recommended applications include first-strand cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP, in vitro transcription and translation, RNA isolation and purification, and RNA sequencing. The product is compatible with reverse transcriptases such as FIREScript® and SOLIScript®, as well as with Taq and Bsm Polymerase, and works efficiently at low DTT concentrations. Stress tests demonstrate tolerance of 60 minutes at 60°C or 1 month at room temperature (25°C), and the formulation is supplied at 220 U/µl. Glycerol-free variants are intended for assays incorporating lyophilization or air-drying, and the stability profile supports dry ice-free shipping.

According to the safety data sheet, the mixture is not classified as hazardous under Regulation (EC) No 1272/2008. It is a colorless liquid with a pH of 7.0–9.0, and the composition includes H2O (<75%), trehalose (<23%), KCl (<1%), Tris-HCl (<1%) and the inhibitor (<0.1%). Routine storage is at -28°C to -18°C until the expiry date marked on the package. The product is for Research Use Only.