HOT FIREPol® DNA Polymerase

Manufactured by Solis BioDyne in compliance with ISO 9001 and ISO 13485 certified quality management systems, this hot-start PCR enzyme is a chemically modified FIREPol® DNA Polymerase designed to improve specificity and accuracy by minimizing mispriming, non-specific primer extension, and primer-dimer formation.

The enzyme is inactive at room temperature and is activated by an initial incubation at 95 °C for 12–15 minutes. It is recommended for routine applications involving fragments up to 3 kb from genomic DNA. The polymerase possesses 5′→3′ polymerase activity, 5′→3′ exonuclease activity, and a non-template-dependent terminal transferase activity, but lacks 3′→5′ exonuclease (proofreading) activity, making the generated product suitable for TA-cloning. Its fidelity is similar to that of a regular Taq DNA Polymerase, with an error rate per nucleotide of ca 2.5 × 10⁻⁵.

The kit includes HOT FIREPol® DNA Polymerase at 5 U/µl in 20 mM Tris-HCl pH 8.7 at 25 °C, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v) and stabilizers, together with 10x Buffer B1 (Mg²⁺ and detergent-free: 0.7 M Tris-HCl, 0.175 M (NH₄)₂SO₄), 10x Buffer B2 (Mg²⁺ free with 0.2% w/v Tween-20), 25 mM MgCl₂, and a 10x GC-rich Enhancer that facilitates amplification of difficult templates such as GC-rich DNA. Reaction setup is at room temperature, shipping is at room temperature, and routine storage is at –20 °C (–28 °C to –18 °C) until the expiry date. The enzyme is stable at 4 °C for 6 months, at 25 °C for 1 month, and tolerates 30 freeze-thaw cycles.