FIREPol® DNA Polymerase

Solis BioDyne offers FIREPol®, a highly processive, thermostable DNA polymerase. Through genetic modifications, the enzyme shows enhanced stability at room temperature with no activity loss for up to 1 month, and both shipping and reaction setup can be performed at room temperature. The polymerase is produced in an E. coli strain carrying an overproducing plasmid with a modified gene of Thermus aquaticus DNA Polymerase. It possesses 5'→3' polymerase and 5'→3' exonuclease activities, as well as non-template-dependent terminal transferase activity, but lacks 3'→5' exonuclease (proofreading) activity, which makes the generated product suitable for TA-cloning.

It is recommended for routine applications, generating fragments up to 3 kb from genomic DNA and 5 kb from low complexity samples such as cDNA or plasmid DNA, and is also suitable for colony PCR. The fidelity is similar to a regular Taq DNA Polymerase, with an error rate per nucleotide of approximately 2.5 × 10⁻⁵.

The kit includes the FIREPol® DNA Polymerase, FIREPol® 10x Buffer B, FIREPol® 10x Buffer BD, 25 mM MgCl₂, and 10x GC-rich Enhancer. Buffer B contains a non-ionic detergent that suppresses inhibitory effects of trace DNA extraction buffer and enhances PCR yield and efficiency. The GC-rich Enhancer is intended for use only when non-specific amplification occurs with difficult templates such as GC-rich DNA. The manufacturer recommends storage at –20°C until the expiry date, with the enzyme remaining stable at 4°C for 6 months and at room temperature (25°C) for 1 month, and withstanding 30 freeze-thaw cycles. Solis BioDyne OÜ manufactures the product in Tartu, Estonia, in compliance with ISO 9001 and ISO 13485 certified quality management systems. The product is supplied for research use only, and FIREPol is a registered trademark of Solis BioDyne OÜ.