FIREPol® DNA Polymerase

A highly processive, thermostable DNA Polymerase from Solis BioDyne, FIREPol® is produced in E. coli carrying an overproducing plasmid with a modified Thermus aquaticus DNA Polymerase gene. The enzyme features enhanced stability at room temperature, with no activity loss for up to 1 month. It exhibits 5'→3' polymerase and 5'→3' exonuclease activity, as well as non-template-dependent terminal transferase activity, but lacks 3'→5' exonuclease (proofreading) activity, making generated products suitable for TA-cloning.

Recommended for routine applications, the enzyme generates fragments up to 3 kb from genomic DNA and 5 kb from low complexity samples such as cDNA and plasmid DNA, and is suitable for colony PCR. Fidelity is similar to that of a regular Taq DNA Polymerase, with an error rate per nucleotide of ca 2.5 x 10^-5.

The kit supplies the enzyme at 5 U/μl in 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers, together with 10x Buffer B (Mg²⁺ free, with detergent: 0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄, 0.2% w/v Tween-20), 10x Buffer BD (Mg²⁺ and detergent free: 0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄), 25 mM MgCl₂, and 10x GC-rich Enhancer for difficult templates such as GC-rich DNA. Storage and handling are flexible: routine storage at –20°C until expiry, with stability at 4°C (2°C to 8°C) for 6 months, at room temperature (25°C) for 1 month, and freeze-thaw stability of 30 cycles. The product is shipped at room temperature, and reaction setup can be performed at room temperature. One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid-insoluble form in 30 minutes at 74°C. It is manufactured by Solis BioDyne in compliance with ISO 9001 and ISO 13485 certified Quality Management System, and is supplied for research use only.