STEMdiff SMADi Neural Induction Kit (x2)

Dual SMAD inhibition provides a rapid and reproducible method for converting human pluripotent stem cells (hPSCs) into neural progenitor cells. This STEMCELL Technologies kit includes the small molecule inhibitors LDN-193189 (BMP pathway inhibitor) and SB-431542 (TGF-β/Activin/Nodal pathway inhibitor), supplied together with neural induction supplements in a two-experiment (x2) format.

The protocol supports both monolayer and embryoid body-based workflows and is compatible with hPSC lines maintained in mTeSR1, mTeSR Plus, or TeSR-E8 systems. Cultures develop PAX6-positive neural rosettes within approximately 7 to 11 days, yielding expandable NPCs that can be further differentiated into neurons, astrocytes, or oligodendrocytes for downstream disease modeling, neurotoxicity screening, and developmental studies.

Each kit contains sufficient reagents for two complete inductions. The small molecule inhibitors should be stored at -20 °C to preserve activity, while the basal medium component is refrigerated and protected from light. For research use only; not intended for diagnostic or therapeutic applications.