EasySep CD4+CD127loCD49d- Kit

From STEMCELL Technologies, this kit enables the isolation of highly purified human CD4+CD127lowCD49d- T cells from peripheral blood mononuclear cells (PBMCs) using the EasySep™ platform. The workflow uses negative selection: unwanted non-CD4, CD127high, and CD49d+ cells are bound by antibody complexes together with dextran-coated magnetic particles and removed using an EasySep™ magnet, without the need for columns. The desired CD4+CD127lowCD49d- cells are simply poured off into a new tube for collection.

According to the manufacturer's profile, when starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%, with donor-to-donor variability noted as unavoidable. A representative example shown by the manufacturer reports purities of 1.84% for the start fraction and 84.1% for the enriched fraction.

The kit is part of the EasySep™ line of column-free immunomagnetic separation products from STEMCELL Technologies and is described as intended for research use. Related workflow components mentioned by the manufacturer include EasySep™ cell separation buffer, magnets such as "The Big Easy" and RoboSep-S, and compatible antibodies for purity assessment of isolated cells. A mouse monoclonal IgG2b antibody against human, rhesus, and cynomolgus CD4 is referenced in the supporting information.