STEMdiff SMADi Neural Induction Kit

From STEMCELL Technologies, this neural induction kit is used to differentiate induced pluripotent stem cells (iPSCs) into neural progenitor cells (NPCs) using a monolayer protocol, as documented in a published working protocol. In the referenced workflow, iPSCs are resuspended in STEMdiff Neural Induction Medium supplemented with SMADi and 10 micromolar (µM) Y-27632 ROCK inhibitor to a final concentration of 1×10^6 cells/mL, then seeded at 2 mL per well (2×10^6 cells/well) of Matrigel- or Geltrex-coated 6-well plates. Cultures receive a daily full-medium change with STEMdiff Neural Induction Medium supplemented with SMADi for 6 days before being split on day 7 (DIV 7) with Accutase. A second seeding and 6-day feeding cycle is performed, followed by an additional split on DIV 13. On DIV 20, NPCs are harvested and either frozen at 2-4×10^6 cells per cryotube using commercial freezing medium or STEMdiff Neural Induction Medium with SMADi plus 10% DMSO, or carried forward into forebrain neuron differentiation.

The kit forms part of a three-stage workflow for producing iPSC-derived forebrain-type neurons, used alongside the STEMdiff Forebrain Neuron Differentiation Kit and the STEMdiff Forebrain Neuron Maturation Kit, both also from STEMCELL Technologies.