STEMdiff Sensory Neuron Mat Kit

From STEMCELL Technologies, the STEMdiff™ Sensory Neuron Maturation Kit is a serum-free medium kit used to mature sensory neuron precursors into functional peripheral sensory neurons capable of long-term culture (> 30 days). The kit includes BrainPhys™ Neuronal Medium as the basal medium, providing a physiological environment for neuronal maturation. The maturation kit is part of the STEMdiff™ Sensory Neuron System and is used in conjunction with the STEMdiff™ Sensory Neuron Differentiation Kit, which generates sensory neuron precursors from human pluripotent stem cell (hPSC)-derived neural crest cells (NCCs).

Sensory neurons can be generated in 7 days from sensory neuron precursors after 6 days in STEMdiff™ Sensory Neuron Differentiation Medium. The resulting cell populations show BRN3A expression (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) and the neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Between Day 12 - 14, more than 70% of cells express class III β-tubulin and peripherin. These functional human PSC-derived sensory neurons respond to sensory ligands such as capsaicin and temperature changes, and are useful for drug discovery and pain research applications, including studies of nociception and thermosensation.