STEMdiff Midbrain Neuron Diff Kit

From STEMCELL Technologies, this differentiation kit generates midbrain neuronal precursors from neural progenitor cells (NPCs) derived from human pluripotent stem cells (hPSCs) using the embryoid body or monolayer protocol following neural induction with the STEMdiff™ SMADi Neural Induction Kit. The resulting precursors can be further matured into midbrain neurons with the STEMdiff™ Midbrain Neuron Maturation Kit.

The culture system produces a population of midbrain neurons containing ≥ 15% TH-positive dopaminergic neurons, ≥ 90% class III β-tubulin-positive neurons, and < 10% GFAP-positive astrocytes. These cells are intended for research applications including modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.

Following the embryoid body protocol, midbrain-type neural precursors can be generated in 18 - 19 days from hPSC-derived NPCs after selecting neural rosettes from replated embryoid bodies. Matured neurons can be maintained in culture for up to 5 weeks. Differentiation may be assessed by immunocytochemistry using antibodies selective for the general neuronal marker β-tubulin III and the dopaminergic marker tyrosine hydroxylase. This product is for research use only.