STEMdiff Lung Progenitor Kit

Designed by STEMCELL Technologies, the STEMdiff™ Lung Progenitor Kit is a serum-free culture medium system for the efficient and reproducible generation of lung progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Differentiation proceeds through three stages — definitive endoderm, anterior foregut endoderm, and lung progenitor cells — and the resulting cells express NKX2.1, a key marker of lung progenitor cells. The lung progenitor cells obtained can be further matured towards proximal or distal airway cells using published protocols, supporting studies of lung development and lung disease.

The system has been optimized for use with mTeSR™1. In the workflow, ES or iPS cell clumps are first seeded in mTeSR™1 or mTeSR™ Plus. On Day 1, differentiation is initiated with Medium DE-1 (Endoderm Basal Medium containing Definitive Endoderm Supplement MR and Supplement CJ). On Days 2 and 3, the medium is changed to Medium DE-2 (Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, the endoderm monolayer is passaged in Medium LP-1 (Lung Basal Medium, Lung Supplement, and Lung Progenitor Supplement 1) with Y-27632 to initiate anterior foregut endoderm patterning. From Day 7, cells are differentiated into the lung progenitor stage with Medium LP-2 (Lung Basal Medium, Lung Supplement, and Lung Progenitor Supplement 2).