STEMdiff Kidney Organoid Kit

Generate three-dimensional kidney tissue from human pluripotent stem cells (hPSCs) using the STEMdiff platform from STEMCELL Technologies.

This directed differentiation system supports generation of organoids containing nephron-like structures, including podocytes, proximal tubules, and collecting duct epithelium. The workflow proceeds through intermediate mesoderm specification via a multi-stage protocol that begins with hPSC maintenance and advances through patterned induction steps.

Researchers typically apply this system for:

• Disease modeling of inherited renal disorders
• Nephrotoxicity screening and drug safety assessment
• Developmental biology studies investigating renal lineage commitment

Each kit provides defined, serum-free media supplements that reduce the variability commonly seen with undefined culture systems. The protocol spans approximately 21 days, with cell aggregation, patterning, and maturation phases requiring sequential medium exchanges.

Components are formulated for storage at -20 °C to preserve cytokine activity across multiple freeze-thaw cycles. The workflow is intended for in vitro research use only and supplied as a single-use kit format suitable for routine organoid generation.