STEMdiff Cardio Dissociation Kit

Enzymatic dissociation of human pluripotent stem cell-derived cardiomyocyte aggregates enables reproducible downstream workflows including flow cytometry, single-cell RNA sequencing, electrophysiology, and cryopreservation. The STEMdiff Cardio Dissociation Kit provides a balanced combination of proteolytic and collagenolytic enzymes optimized for the STEMCELL Technologies cardiac differentiation protocol.

The reagents preserve key cardiac markers such as cTnT and alpha-actinin while maintaining high viability and yield, completing a typical dissociation within 15-30 minutes at 37 degrees C. This gentle approach minimizes mechanical stress on beating cardiomyocytes and supports successful replating for further maturation studies or downstream in vitro assays.

Packaged as a single-use reagent set, the kit is intended for research use only and integrates directly with engineered cardiac tissue workflows. Investigators focused on cardiac disease modeling, drug screening, and regenerative medicine apply the dissociation reagents to generate reproducible single-cell preparations from both 2D differentiation cultures and 3D cardiac microtissues, with the resulting suspension suitable for immunostaining, patch-clamp recording, and reaggregation experiments.