RosetteSep Human NK Enrichment, 2 mL

From STEMCELL Technologies, this enrichment cocktail isolates NK cells from human whole blood by negative selection. The cocktail contains a combination of monoclonal antibodies in PBS, formulated as bispecific Tetrameric Antibody Complexes (TACs) directed against cell surface antigens on non-NK cells. When added to whole blood, unwanted cells are crosslinked to multiple red blood cells to form immunorosettes. During centrifugation over a density gradient medium, the rosetted cells pellet with the free RBCs, while desired NK cells are never labeled and are easily collected as an enriched population at the plasma–density gradient interface.

Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction. The workflow requires no special equipment or training, and the product can be combined with SepMate™ tubes for consistent, high-throughput sample processing. The recommended medium is PBS with 2% fetal bovine serum; compatible density gradient media include Lymphoprep™ and similar media with a density of 1.077 g/mL. Starting with fresh human whole blood, the CD56+ content of the enriched fraction typically ranges from 80 - 98%, with a representative example showing 7% CD56+ cells at start rising to 94% after enrichment.

The cocktail is supplied in PBS and should be stored at 2 - 8°C; it should not be frozen, and may be shipped at room temperature. Precipitate may be observed in the vial but does not affect performance. For research use only.