RosetteSep Human NK Enrichment, 10 mL

Manufactured by STEMCELL Technologies, this enrichment cocktail isolates NK cells from human whole blood by negative selection. The antibody cocktail crosslinks unwanted cells in whole blood to multiple red blood cells (RBCs), forming immunorosettes that pellet along with free RBCs during density gradient centrifugation. The desired NK cells remain unlabeled and are collected as a highly enriched population at the plasma–density gradient medium interface. The procedure requires no special equipment or training, and isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

Starting with fresh human whole blood, the CD56+ cell content of the enriched fraction typically ranges from 80 – 98%. The cocktail contains a combination of monoclonal antibodies in PBS and should be stored at 2 – 8°C; it should not be frozen, though it may be shipped at room temperature (15 – 25°C). A precipitate may be observed in the vial but will not affect performance. For more rapid processing, the product can be combined with SepMate™ tubes for consistent, high-throughput sample preparation.

For use, the cocktail is added to whole blood at 50 µL/mL and incubated for 20 minutes at room temperature (15 – 25°C). The sample is then diluted with an equal volume of recommended medium (PBS + 2% FBS), layered over density gradient medium, and centrifuged at 1200 x g for 20 minutes. Enriched cells are harvested from the density gradient medium:plasma interface, washed, and used as desired. The product is for research use only and is not intended for human or animal diagnostic or therapeutic uses.