RosetteSep Human CD4+ Enrichment, 10 mL

Designed to isolate CD4+ T cells from human whole blood by negative selection, this immunodensity-based enrichment cocktail from STEMCELL Technologies is fast and easy to use. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes that recognize non-CD4+ T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium, the unwanted cells pellet along with the RBCs, leaving the purified CD4+ T cells as a highly enriched population at the interface between the plasma and the density medium. According to the manufacturer, the CD4+ (CD3+CD4+) T cell content of the enriched fraction is typically 94.7 ± 2.4% (mean ± SD), and in a FACS example the start and final enriched fractions measured 15.1% and 95.0%, respectively. The antibody component is a mouse monoclonal IgG2b directed against human, rhesus, and cynomolgus CD4.

The workflow requires no special equipment or training, leaves isolated cells untouched, and can be combined with SepMate™ for consistent, high-throughput sample processing. The product is intended for immunology research and cell-isolation applications and is offered in a tube format suitable for density gradient centrifugation in in vitro diagnostic (IVD) use. Acceptable sample sources include whole blood and buffy coat.