RosetteSep Human CD4 Depletion, 2mL

A depletion cocktail from STEMCELL Technologies designed to remove CD4+ cells directly from human whole blood, leaving desired cells untouched and viable. The reagent is fast and easy-to-use, requires no special equipment or training, and can be combined with SepMate™ for consistent, high-throughput sample processing.

The cocktail contains a combination of monoclonal antibodies in PBS that crosslink unwanted CD4+ cells in whole blood to multiple red blood cells (RBCs) via Tetrameric Antibody Complexes recognizing CD4 and glycophorin A, forming immunorosettes. When centrifuged over a buoyant density medium, the rosetted unwanted cells pellet along with the free RBCs, while desired cells are easily collected as a highly enriched population at the interface between the plasma and the density gradient medium. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

For optimal performance, whole peripheral blood should be used within 24 hours of collection and stored at room temperature (15 - 25°C), with the nucleated cell concentration not exceeding 5 x 10^7 cells/mL and at least 100 RBCs per nucleated cell. The recommended density gradient medium is Lymphoprep™ (1.077 g/mL) or equivalent, and the recommended wash medium is Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum. The cocktail should be stored at 2 - 8°C without freezing and is stable until the expiry date on the label. According to the manufacturer's typical data, CD4+ content can be reduced from 12% in the start fraction to 1.6% in the depleted fraction, achieving a 2.0 log depletion of CD4+ cells.