RosetteSep Human CD3 Depletion, 10mL

STEMCELL Technologies' RosetteSep™ Human CD3 Depletion Cocktail is an immunodensity depletion cocktail designed to deplete CD3+ cells directly from human whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3 and glycophorin A on red blood cells (RBCs). The antibody cocktail crosslinks unwanted cells to multiple RBCs, forming immunorosettes that pellet along with free RBCs when centrifuged over a buoyant density medium, leaving desired cells as a highly enriched population at the interface between the plasma and the density gradient medium.

The protocol is fast and easy-to-use, requires no special equipment or training, and yields untouched, viable cells. It can be combined with SepMate™ tubes for consistent, high-throughput processing, and can be performed on whole blood or buffy coat provided that RBCs are present at a ratio of at least 100 RBCs per nucleated cell. Isolated cells are immediately suitable for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

For optimal performance, whole peripheral blood should be used within 24 hours of collection and stored at room temperature. The cocktail is added at 50 µL/mL of sample, and the nucleated cell concentration should not exceed 5 x 10^7 cells/mL. Recommended density gradient medium has a density of 1.077 g/mL. The product is intended for research use only.