RosetteSep HLA T Enrich Cocktail, 40mL

From STEMCELL Technologies, this cell enrichment cocktail is designed for the in vitro enrichment of T cells from human whole blood by negative selection. The antibody cocktail crosslinks unwanted cells to multiple red blood cells (RBCs), forming immunorosettes. This increases the density of the unwanted (rosetted) cells, such that they pellet along with the free RBCs when centrifuged over a density gradient medium. Desired T cells are never labeled with antibody and are easily collected as a highly enriched population at the interface between the plasma and the density gradient medium.

The workflow requires no special equipment or training, and isolated cells are described as untouched and immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction, and as ready for use in serology or flow cytometry crossmatch assays. Typical purity of the CD3+ enriched fraction from fresh whole blood is reported at >90% (typically ranging from 90% to 97%). The product is a combination of monoclonal antibodies in PBS, is stored at 2 - 8°C, and each lot is sterility tested according to USP methods.

For use, the cocktail is added at 40 µL per mL of whole blood and incubated at room temperature (15 - 25°C) for 20 minutes, then processed over a density gradient medium with a density of 1.077 g/mL by centrifugation at 1200 x g for 20 minutes with the brake off. It can be combined with SepMate™ for consistent, high-throughput sample processing.