RosetteSep HLA T Enrich Cocktail, 10mL

From STEMCELL Technologies, the RosetteSep™ HLA T Cell Enrichment Cocktail enriches untouched and highly purified T cells directly from human whole blood by negative selection. The antibody cocktail targets non-T cells for removal by crosslinking unwanted cells in whole blood to multiple red blood cells (RBCs), forming immunorosettes that pellet with free RBCs during density gradient centrifugation. Desired T cells are never labeled with antibody and are collected as a highly enriched population at the plasma–density gradient interface, ready for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

Designed for fast, easy use without special equipment or training, the cocktail is optimized for use in downstream HLA tests, including the complement-dependent cytotoxicity (CDC) and flow cytometric crossmatch (FCXM) assays, and supports workflows for HLA crossmatch analysis, serological typing, and chimerism analysis. It can be combined with SepMate™ for consistent, high-throughput sample processing. Each lot is manufactured using aseptic technique, sterility tested according to USP methods, and quality control performance tested in cell separation assays using human whole blood.

Typical usage involves adding the cocktail at 40 μL/mL of whole blood, incubating for 20 minutes at room temperature (15 - 25°C), then layering the diluted sample over a density gradient medium and centrifuging at 1200 x g for 20 minutes at room temperature. The product is a combination of monoclonal antibodies in PBS and should be stored at 2 - 8°C without freezing. T cell (CD3+) purity of the enriched fraction is reported as > 90% in the product specifications, with a typical range of 90% - 97%.