RosetteSep Bone Marrow Enrichment, 10 mL

This negative selection cocktail from STEMCELL Technologies enriches untouched bone marrow progenitor cells from human whole bone marrow. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD11b, CD14, CD16, CD19, CD56, CD66b and glycophorin A on red blood cells (RBCs). The cocktail crosslinks these unwanted cells to multiple RBCs to form immunorosettes, increasing their density. When centrifuged over a buoyant density medium such as Lymphoprep™, the rosetted unwanted cells pellet along with the free RBCs, while the unlabeled desired cells are easily collected as a highly enriched population at the interface between the plasma and the density gradient medium.

The kit is fast and easy-to-use, requires no special equipment or training, and yields untouched, viable cells that are immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction. It can be combined with SepMate™ for consistent, high-throughput sample processing.

The cocktail is supplied as a combination of monoclonal antibodies in PBS. It should be stored at 2 - 8°C, not frozen, and is stable until the expiry date on the label. The recommended density gradient medium has a density of 1.077 g/mL, and the concentration of nucleated cells in the sample should not exceed 5 x 10^7 cells/mL. Starting with whole bone marrow, the bone marrow progenitor cell enrichment of the isolated fraction is typically 25 ± 10-fold. Reported purity values for the start and final isolated fractions are 0.6% and 17%, respectively (29-fold enrichment of CD34+ cells), with a Fold Enrichment of CFC of 10-53 Fold and Enrichment of CD34+ Cells of 25 ± 10%.