RoboSep Ser WB CD19 Pos Slctn Kit

From STEMCELL Technologies, this kit enables isolation of highly purified CD19+ cells from fresh human whole blood by immunomagnetic positive selection. The workflow is designed for compatibility with HLA serology and requires no columns. Desired cells are labeled with antibodies and magnetic particles and separated using an EasySep™ magnet, so unwanted cells are simply poured off while desired cells remain in the tube. The protocol is described as fast and easy to use, and the RoboSep™ automated format is supported alongside manual processing on "The Big Easy" and EasyEights™ magnets.

Isolated cells are immediately available for downstream applications such as HLA serology, flow cytometry, or DNA/RNA extraction. The published performance claim cites up to 98% purity. In the manufacturer's representative data, starting with human whole blood and using "The Big Easy" EasySep™ Magnet, the CD19+ cell content of the isolated fraction was typically 93.6 ± 6.2% (mean ± SD), with start and final isolated fraction purities of 1.1% and 95.1%, respectively, in the example shown. Red blood cells were removed from the start sample by lysis prior to flow cytometry.

The kit comprises a Positive Selection Cocktail containing a combination of monoclonal antibodies in PBS and 2% HPCD (2-hydroxypropyl-β-cyclodextrin), including an Fc receptor blocking antibody, together with Releasable RapidSpheres™, a suspension of magnetic particles in water. Both components are stored at 2 - 8°C and remain stable until the expiry date on the label. Recommended media are EasySep™ Buffer, RoboSep™ Buffer, or PBS containing 2% fetal bovine serum (FBS) and 1 mM EDTA, free of Ca++ and Mg++. Products are for research use only and are not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.