RoboSep Release Hu CD4 Pos Slctn Kit

Designed by STEMCELL Technologies, this kit enables the isolation of highly purified human CD4+ cells from fresh or previously frozen PBMCs or washed leukapheresis samples. Using the optimized EasySep™ protocol, isolations can be completed in as little as 30 minutes via column-free immunomagnetic positive selection.

When starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep™ Magnet). In one reported example, the start and final isolated fractions measured 24.0% and 98.1% purity, respectively. The kit includes a Mouse (BALB/c) monoclonal IgG1 antibody against human and chimpanzee CD3, a Mouse monoclonal IgG2b antibody against human, rhesus, and cynomolgus CD4, and a Mouse monoclonal IgG1 antibody against human, rhesus, and cynomolgus CD8a.

Directions for use are available in the Product Information Sheet, and compatible accessories such as a magnet, cell separation buffer, and antibodies for purity assessment are offered separately.