RoboSep Release Hu CD3 Pos Slctn Kit

This immunomagnetic positive selection kit from STEMCELL Technologies uses particle release technology to isolate highly purified, magnetic particle-free human CD3+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples in less than 30 minutes. Combining the specificity of monoclonal antibodies with a column-free magnetic system, desired cells are labeled with antibody complexes recognizing CD3 and with EasySep™ Releasable RapidSpheres™. After separation in an EasySep™ magnet, the magnetic particles are released from the isolated CD3+ cells using a release agent, while unwanted non-CD3+ cells are targeted for depletion, yielding a fraction immediately ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Starting from a single-cell suspension of human PBMCs, the CD3+ cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD) using the purple EasySep™ Magnet, with example start and final isolated fraction purities of 38.4% and 99.0%, respectively. The CD3 antigen is expressed on all T cells and CD56+ NKT cells, and the kit is suited to cell therapy research applications, where it may be qualified as an ancillary material under the USP<1043> framework. Following isolation, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.