RoboSep Negative Human NK

The EasySep™ Human NK Cell Enrichment Kit from STEMCELL Technologies uses immunomagnetic negative selection to isolate untouched human natural killer (NK) cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples. Widely used in published research for more than 20 years, the kit combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this negative selection procedure, unwanted cells—including granulocytes, T cells, B cells, monocytes, dendritic cells, and erythroid cells—are labeled with antibody complexes and magnetic particles and then separated from the desired untouched human NK cells by using an EasySep™ magnet and pouring or pipetting the desired cells into a new tube. The RoboSep™ format of the kit includes filter tips for automated handling. Starting with previously frozen mononuclear cells containing more than 10% NK cells, the NK cell content of the enriched fraction typically ranges from 73% to 95%, with purities that may be lower when starting with samples containing less than 10% NK cells.

Following magnetic isolation, the desired human NK cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.