RoboSep Mouse CD138 Pos Selection Kit

From STEMCELL Technologies, this immunomagnetic positive selection kit isolates highly purified CD138+ cells from mouse splenocytes, lymph nodes, or bone marrow using a column-free workflow. Desired cells are labeled with monoclonal antibodies and magnetic particles, then separated with a compatible magnet while unwanted cells are poured off, leaving the cells of interest in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, and hybridoma generation.

The kit is designed for the enrichment of plasma cells and achieves up to 97% purity with immunized mice. It is also compatible with hybridoma generation protocols, including electrofusion, and is for processing 2 x 10^9 cells.

In typical performance data, starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD) and the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3%. Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content is typically 78.3 ± 5.7% and the plasma cell content is typically 50.8 ± 10.0%. When isolated CD138+ cells were fused with Sp2/0 mouse myeloma cells in a hybridoma workflow, the antigen-specific hit rate was 28.8 ± 11.0% and the percentage of antibody-secreting hybridomas reached 99.3 ± 0.6%.