RoboSep Human Naive CD8+ T Cell Iso Kit

This immunomagnetic negative selection kit from STEMCELL Technologies enables researchers to efficiently isolate highly purified human naïve CD8+ T cells (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) from fresh peripheral blood mononuclear cell (PBMC) samples. Part of the EasySep™ product line, this column-free system combines the specificity of monoclonal antibodies with the simplicity of a magnetic separation approach. Unwanted cells are labeled with antibody complexes and magnetic particles, while the desired naïve CD8+ T cells remain untouched and viable for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

The kit targets a broad panel of unwanted cells for removal, including those expressing CD4, CD14, CD16, CD19, CD20, CD36, CD45RO, CD56, CD57, CD94, CD123, CD244, TCRγ/δ, and glycophorin A. EasySep™ technology has been widely used in published research for more than 20 years. When starting with fresh PBMCs, the naïve CD8+ T cell content of the isolated fraction is typically 92.3 ± 4.0% (mean ± SD) using the purple EasySep™ Magnet, with the start and final isolated fractions reported at 3.9% and 92.8%, respectively. The kit is noted to deliver up to 97% purity and is compatible with various EasySep™ magnets, including RoboSep™-S for automated workflows.