RoboSep Human CD3 Pos Sel Kit II

An immunomagnetic positive selection kit from STEMCELL Technologies, the RoboSep™ Human CD3 Positive Selection Kit II isolates CD3+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 together with dextran-coated magnetic particles, and the cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding.

Labeled cells are separated using an EasySep™ magnet without the use of columns, so cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on all T cells and CD56+ NKT cells. In typical FACS analysis using the purple EasySep™ Magnet, the CD3+ cell content of the isolated fraction is 99.2 ± 0.2% (mean ± SD).

Designed to be fast and easy to use with no columns required, the kit is suited to cell separation work in T cell, immunology, and chimerism research. It is supplied with the EasySep™ Human CD3 Positive Selection Cocktail II, EasySep™ Dextran RapidSpheres™ 50100, RoboSep™ Buffer, and RoboSep™ Filter Tips, and is compatible with the EasySep™ Magnet, "The Big Easy" EasySep™ Magnet, EasyPlate™ EasySep™ Magnet, EasyEights™ EasySep™ Magnet, Easy 50 EasySep™ Magnet, and the RoboSep™-S instrument.