RoboSep Hu Progenitor Enrichment Kit II

This immunomagnetic negative selection kit from STEMCELL Technologies is designed to isolate untouched and highly purified hematopoietic progenitor cells from fresh human cord blood. The column-free procedure uses antibody complexes and magnetic particles to label unwanted cells, which are then separated using an EasySep™ magnet; the desired cells are simply poured off into a new tube and are immediately available for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or adoptive transfer into mice.

The kit targets non-progenitor cells for removal using antibodies that recognize specific cell surface markers and is rated for processing up to 1 x 10^9 cells. Reported purity of the isolated fraction is up to 95%. In typical use with 36- to 48-hour-old cord blood, the CD34+ cell content of the isolated fraction is 77.5 ± 16.0% (mean ± SD), with start and final isolated fraction purities of 0.7% and 82.4%, respectively. The workflow is compatible with the EasySep™ magnet, "The Big Easy" EasySep™ Magnet, and the RoboSep™-S instrument.

Components include the EasySep™ Human Progenitor Cell Enrichment Cocktail, a combination of monoclonal antibodies in PBS, and EasySep™ Dextran RapidSpheres™, a suspension of magnetic particles in water. Both should be stored at 2 - 8°C without freezing and are stable until the expiry date on the label. Recommended media include EasySep™ Buffer, RoboSep™ Buffer, or PBS containing 2% fetal bovine serum and 1 mM EDTA, free of Ca++ and Mg++.