RoboSep Hu Memory CD8 T Cell Enrich Kit

The immunomagnetic cell separation kit from STEMCELL Technologies is designed to isolate untouched and highly purified memory CD8+ T cells (CD8+CD45RA-CD45RO+) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by negative selection, with capacity for processing 1 x 10^9 cells. Unwanted cells are targeted for removal with antibody complexes recognising CD4, CD14, CD16, CD19, CD20, CD34, CD36, CD45RA, CD56, CD61, CD66b, CD123, TCRγ/δ, and glycophorin A, along with dextran-coated magnetic particles. Labelled cells are separated without columns using an EasySep™ magnet, and the desired cells are simply poured off into a new tube and used directly in downstream applications such as flow cytometry, culture, or cell-based assays.

The workflow is fast, easy-to-use, and column-free, delivering up to 92% purity with untouched, viable cells. Starting with mononuclear cells, the memory CD8+ T cell content of the enriched fraction typically ranges from 72 - 92%; in the manufacturer's example, purities of the starting and enriched fractions were 4.9% and 88.8%, respectively. This format is configured for use with the RoboSep™ fully automated cell separator.

Kit components should be stored at 2 - 8°C and not frozen; they are stable until the expiry date on the label. Components may be shipped at room temperature (15 - 25°C).