RoboSep Hu B Cell EnrKitII w/o CD43 Dep

This immunomagnetic negative selection kit from STEMCELL Technologies enables isolation of untouched, highly purified human B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs). It is intended for samples from individuals with B cell leukemia or lymphoma, or other diseases in which B cells may express CD43, and uses column-free magnetic separation compatible with EasySep™ magnets or the fully automated RoboSep™ system.

The kit targets non-B cells for removal with antibodies recognizing specific cell surface markers. Unwanted cells are labeled with antibodies and magnetic particles, while desired B cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Up to 99% purity is reported, and starting with fresh PBMCs the B cell content (CD19+) of the enriched fraction is typically 84.9 ± 13.9% (mean ± SD using the purple EasySep™ Magnet).

Components include a combination of monoclonal antibodies in PBS, an isolation cocktail enhancer solution, and a suspension of magnetic particles in water, all stored at 2-8°C and stable until the expiry date on the label. For research use only.