ImmunoCult DC Culture Kit

STEMCELL Technologies offers a complete system for the in vitro culture and differentiation of human monocytes into dendritic cells (DCs) and their subsequent maturation into fully active mature DCs. The system enables users to generate active mature DCs from human monocytes in 7 days using an animal component-free (ACF) medium.

The kit includes ImmunoCult™-ACF Dendritic Cell Medium (100 mL), ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (1 mL), and ImmunoCult™ Dendritic Cell Maturation Supplement (0.5 mL). The medium is serum-free and animal component-free, containing recombinant proteins and synthetic components, with no serum or other human- or animal-derived components. Recombinant growth factors are not added to the medium, allowing users the flexibility to prepare a medium that meets their requirements. The Differentiation Supplement contains a combination of animal component-free recombinant human cytokines formulated to support the differentiation of DCs from human monocytes and is supplied as a 100X concentrate. The Maturation Supplement is formulated to support the maturation of immature DCs and is also supplied as a 100X concentrate. The medium should be stored at 2 - 8°C and the supplements at -20°C; all components are stable for 1.5 years from the date of manufacture.

In performance data reported by the manufacturer, 93 ± 5% of cells expressed the mature DC marker CD83 at day 7, with only 1 ± 1% still expressing the monocyte marker CD14, and the yield of viable mature DCs corresponded to 45 ± 25%. IL-12p70 concentrations in supernatant of mature and immature DCs were 361 ± 81 and 5 ± 2 pg/mL, respectively. STEMCELL Technologies' quality management system is certified to ISO 13485 medical device standards. The product is for research use only and not intended for human or animal diagnostic or therapeutic uses.