HepatiCult OGM Mouse Kit

A serum-free, defined cell culture medium from STEMCELL Technologies is designed for the establishment and maintenance of mouse hepatic progenitor organoids. These organoids, referred to as "mini-livers," provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells.

The organoids feature an epithelium expressing markers of hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP). Hepatic organoids can be passaged every 4 - 7 days, can be cryopreserved, and are primed for downstream differentiation.

The medium supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use.