ES Rel Hu CD45 Pos Slctn Kit for Hu Mice

Recover viable human CD45+ leukocytes from humanized mouse tissues using this positive selection kit from STEMCELL Technologies. The immunomagnetic workflow targets the human CD45 pan-leukocyte marker expressed on nucleated hematopoietic cells, then removes the magnetic particles from the cell surface with the included Releasase enzyme. The result is a purified, bead-free population of human immune cells ready for downstream analysis.

This kit is a common tool in humanized mouse research where immunodeficient strains such as NSG or NOG are engrafted with human hematopoietic stem cells. After tissue dissociation from spleen, bone marrow, or peripheral blood, researchers use the kit to monitor engraftment levels, harvest specific human immune subsets, or prepare leukocytes for xenograft, immunotherapy, and infectious disease studies.

Downstream applications include flow cytometry, adoptive transfer, in vitro culture, cytotoxicity assays, proliferation studies, and cytokine profiling. The antibody cocktail specifically recognizes human CD45 and exhibits no significant cross-reactivity with mouse CD45, ensuring clean separation of human cells from mouse background. The standard protocol completes in under one hour using standard laboratory equipment, and kit components should be refrigerated to preserve reagent activity.