EasySepMsCD4+CD25+RegTCell IsoKitII-Sam

Column-free immunomagnetic isolation of mouse CD4+CD25+ regulatory T cells from single-cell suspensions of spleen, lymph node, or peripheral blood. The kit from STEMCELL Technologies relies on a biotinylated antibody cocktail directed against non-target cells together with magnetic dextran-iron particles; unwanted cells are rosetted and retained in a magnet while the Treg fraction flows through into the collection tube. Recovered Tregs typically exceed 85% purity by flow cytometry and are immediately ready for downstream in vitro suppression assays and adoptive transfer studies in mouse models of autoimmunity and transplant tolerance. The small-sample format supplies single-use vials sized for one mouse spleen, and components should be refrigerated and protected from light to preserve antibody activity.