EasySepHLA Chim BC CD14 Pos Slctn Kit

This immunomagnetic positive selection kit from STEMCELL Technologies isolates highly purified human CD14+ cells from a leukoreduction system chamber (LRSC), also known as an LRS cone. Target cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet — unwanted cells are simply poured off while desired cells remain in the tube.

Key features include up to 99% purity, no columns required, and compatibility across EasySep™ platforms including "The Big Easy", EasyEights™, and the RoboSep™-S. The kit is supplied with a Selection Cocktail (a combination of monoclonal antibodies in PBS), EasySep™ Dextran RapidSpheres™ (a suspension of magnetic particles), and EasySep™ Red Blood Cell Lysis Buffer (a 10X concentrated reagent). Samples are processed at volumes between 1 - 4.5 mL, and isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Starting with LRSC samples, the CD14+ cell content of the isolated fraction typically ranges from 91.1 - 99.5%. With buffy coat starting material, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using "The Big Easy" EasySep™ Magnet).