EasySep SER WB CD3

STEMCELL Technologies' EasySep™ Serology Whole Blood CD3 Positive Selection Kit is designed to isolate highly purified CD3+ cells from fresh human whole blood by immunomagnetic positive selection. The kit targets human CD3+ cells for positive selection.

In the cited workflow example, the CD3+ cell content of the isolated fraction typically ranges from 96.7 - 99.6%. Note that, as the CD3 antibody used for flow cytometry staining was fully blocked by the CD3 antibody used to isolate the cells, staining of T cells was assessed by labeling with anti-CD2; gated on CD45+. In this example, the purities of the start and final isolated fractions are 25.4% and 98.7% (gated on CD45+), respectively.

Red blood cells were removed by lysis prior to flow cytometry. The magnetic particles present on the surface of the isolated CD3+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer, making it difficult to gate on a specific lymphocyte population based on FSC/SSC; gating on CD45 and examining the lymphocyte populations based on the CD45+ gate is recommended. The product is for research use only and not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.