EasySep Release Hu Biotin Pos Slctn Kit

From STEMCELL Technologies, this immunomagnetic positive selection kit enables isolation of magnetic particle-free human cells labeled with biotinylated antibodies from PBMCs and washed leukapheresis samples, and is supplied for processing 1 x 10^9 cells. Selection uses EasySep™ Releasable RapidSpheres™ that bind to biotinylated antibody-labeled cells; the particles are subsequently released so that isolated cells are free of magnetic particles. Cells are separated without columns using an EasySep™ magnet, and unwanted cells are simply poured off while desired cells remain in the tube.

Highly purified cells can be obtained in less than 40 minutes and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Starting with fresh PBMCs, typical purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody (as assessed by labeling with CD45RO and CD45RA). Following isolation, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Components include an EasySep™ Release Biotin Positive Selection Cocktail (a combination of monoclonal antibodies in PBS and 0.1% BSA), EasySep™ Releasable RapidSpheres™ (a suspension of magnetic particles in water), EasySep™ Release Buffer (Concentrate) for release of the RapidSpheres™ from cells after positive selection, and EasySep™ Anti-Human CD32 (Fc gamma RII) Blocker for Positive Selection. All components should be stored at 2 - 8°C and not frozen.