EasySep Ms Pan DC Enrich Kit II-Sam

Mouse dendritic cells (both conventional DCs and plasmacytoid DCs) are enriched from single-cell suspensions of spleen, lymph node, or bone marrow by negative-selection immunomagnetic separation using the EasySep Mouse Pan-DC Enrichment Kit II from STEMCELL Technologies. The cocktail depletes non-DC leukocytes with biotinylated antibodies directed against T cells, B cells, NK cells, monocytes, granulocytes, and erythroid cells. Labeled cells bind streptavidin-coated magnetic particles and are retained inside an EasySep magnet, leaving untouched cDCs and pDCs to flow through with preserved surface markers and viability for downstream cell culture, flow cytometry, and adoptive transfer studies. Researchers apply this approach to antigen presentation assays, vaccine development workflows, autoimmune disease modeling, transplantation immunology, and infectious disease research in mouse models. Reagents are supplied in vials and should be refrigerated at 4 degrees C; protect from light until first use. Each kit is intended for in vitro research use only and provides sufficient antibody cocktail and particles to process approximately 2 x 10^9 total cells, typically yielding greater than 85% CD11c+ purity.