EasySep Mouse Hem Prog Iso Kit-Sam

Isolate mouse hematopoietic progenitor cells from bone marrow, spleen, or fetal liver suspensions with this immunomagnetic separation kit from STEMCELL Technologies. The protocol uses a negative-selection strategy, leaving unwanted lineage-positive cells behind while recovering untouched progenitor populations ready for downstream culture, transplantation, or flow cytometry analysis. Components include a cocktail of biotinylated antibodies targeting non-progenitor lineages, a magnetic particle suspension, and a buffer optimized for the EasySep column-free workflow. The 1 mL antibody vial pairs with a single-use sample of magnetic particles, allowing small-scale processing for pilot experiments and method development before committing to larger prep sizes. Cells are typically processed in a 5 mL polystyrene tube and run on an EasySep magnet; the entire workflow completes in roughly 30 minutes at room temperature and does not require a density-gradient centrifugation step. Researchers working with c-Kit-enriched marrow fractions, transplant reconstitution studies, or ex vivo expansion of colony-forming units will find this preparation a practical starting point for stem and progenitor cell investigations.