EasySep Mouse CD90.1 Pos Selection Kit

From STEMCELL Technologies, the EasySep™ Mouse CD90.1 Positive Selection Kit enables immunomagnetic positive selection of highly purified CD90.1+ (Thy1.1+) cells from mouse splenocytes, lymph nodes, or peripheral blood, and can also be applied to CD90.1+ cells from adoptive cell transfer (ACT) samples. The column-free workflow uses an antibody cocktail that recognizes the CD90.1 surface marker together with magnetic particles, and separation is performed using an EasySep™ magnet by pouring off unwanted cells while the desired cells remain in the tube. Isolated cells are not fluorochrome-labeled and are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.

Reported performance figures from the product documentation state that the kit is for processing 2 x 10^9 cells, with claimed purity of up to 97%. Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD using the purple EasySep™ Magnet), and starting with mouse splenocytes from adoptive cell transfer samples, the CD90.1+ (Thy1.1+) content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD using the purple EasySep™ Magnet). The kit has been verified for use with FVB mouse strains and is expected to be compatible with other Thy1.1+ strains. For purity assessment by flow cytometry, the documentation recommends the Anti-Rat CD90 Antibody, Clone OX-7.

The kit is intended for research use only and is manufactured under an ISO 13485-certified quality management system. Recommended separation media include EasySep™ Buffer, RoboSep™ Buffer, or PBS containing 2% FBS and 1 mM EDTA, with HBSS, Modified (without Ca++ and Mg++) as an alternative to PBS; medium should be free of Ca++ and Mg++.