EasySep Human Pan-B Cell Sample Kit

This column-free negative-selection immunomagnetic kit from STEMCELL Technologies enriches untouched human pan-B lymphocytes from peripheral blood mononuclear cells, whole blood, or leukapheresis samples in approximately 25 minutes. Target B cells remain unlabeled and free of magnetic particles, preserving viability and surface marker expression for downstream assays such as flow cytometry, in vitro culture, and functional immunology studies. The protocol requires only a standard EasySep magnet and reaches >95% purity of CD19+ B cells without the need for density-gradient centrifugation or columns. Antibody cocktail and magnetic particle components are provided in vials sufficient for processing small-volume samples, with components stored refrigerated until first use. Isolated cells are ready for phenotyping, B-cell receptor sequencing, stimulation assays, or co-culture experiments.