EasySep Human Naïve CD8+ T Cell Iso Kit

STEMCELL Technologies' EasySep™ kit enables isolation of highly purified human naïve CD8+ T cells, defined as CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-, from fresh peripheral blood mononuclear cells (PBMCs). The workflow uses column-free immunomagnetic negative selection. Unwanted cells are labeled with an antibody cocktail recognizing CD4, CD14, CD16, CD19, CD20, CD36, CD45RO, CD56, CD57, CD94, CD123, CD244, TCRγ/δ, and glycophorin A together with dextran-coated magnetic particles, then separated with an EasySep™ magnet. Untargeted naïve CD8+ T cells are collected by pouring the suspension into a new tube.

Starting with fresh PBMCs, the naïve CD8+ T cell content of the isolated fraction is typically 92.3 ± 4.0% (mean ± SD) using the purple EasySep™ Magnet; in the cited example, start and final purities were 3.9% and 92.8%, respectively. Compatible accessories include a cell separation buffer and an EasySep™ magnet for column-free immunomagnetic separation. Mouse monoclonal antibodies suitable for downstream purity assessment include IgG1 anti-human CD56 (NCAM), IgG1 anti-human, rhesus, and cynomolgus CD8a, and IgG2a anti-human, chimpanzee, and common marmoset CD45RO. The kit is intended for research use only and is not approved for human or animal medical, clinical diagnostic, or food applications.