EasySep Human CD19 Pos Sel Kit II

From STEMCELL Technologies, this immunomagnetic kit enables positive selection of highly purified CD19+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. The column-free workflow uses antibodies recognizing the CD19 surface marker together with magnetic particles, with separations performed using an EasySep™ magnet. The kit is designed for processing 1 x 10^9 cells and can deliver results in as little as 18 minutes, with up to 99% purity.

The kit includes a Positive Selection Cocktail — a combination of monoclonal antibodies in PBS with 0.09% recombinant human albumin and an Fc receptor blocking antibody — and Dextran RapidSpheres™, a suspension of magnetic particles in water. Both components are stored at 2 - 8°C, must not be frozen, and remain stable until the expiry date on the label. Samples are prepared at 1 x 10^8 cells/mL, and isolated cells are immediately suitable for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Both manual EasySep™ and fully automated RoboSep™ protocols are supported.

Starting with human PBMCs, the CD19+ content of the isolated fraction is typically 98 ± 1% (mean ± SD using the purple EasySep™ Magnet). The same reagent set can also be applied in a depletion protocol, achieving up to 99% depletion of CD19+ cells in as little as 18 minutes.