EasySep Hu T Cell Iso Kit, 10 mL

Manufactured by STEMCELL Technologies, this kit enables researchers to isolate untouched, highly purified human T cells by immunomagnetic negative selection. Unwanted non-T cells are labeled with a cocktail of monoclonal antibodies and magnetic particles, then separated without the use of columns; the desired T cells are simply pipetted off into a new vessel. The procedure is column-free and can be completed in as little as 8 minutes.

The kit is intended for processing large leukapheresis samples and is designed for use with the Easy 250 EasySep™ Magnet. Two components are supplied: an EasySep™ Human T Cell Isolation Cocktail (a combination of monoclonal antibodies in PBS) and EasySep™ Dextran RapidSpheres™ (a suspension of magnetic particles in water), both stored at 2 - 8°C. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Starting from human peripheral blood mononuclear cells (PBMCs), the T cell content (CD3+) of the isolated fraction is typically 96.7 ± 1.5% (mean ± SD). When starting with washed or lysed leukapheresis samples, the CD3+ T cell content of the isolated fraction is typically 96.5 ± 2.6% (mean ± SD for the Easy 250 EasySep™ Magnet). Reported purity reaches up to 99% with high recovery. For research use only; not intended for human or animal diagnostic or therapeutic uses.